Want to know more about your antibodies?  From mRNA to protein sequence…let our experts do the decoding! 

Our molecular biology services:

• Messenger RNA isolation from hybridoma cells.
• DNA sequencing of immunoglobulin heavy and light chain variable regions.
• Edman Degradation for sequence confirmation.
• Cloning of variable region DNA into vectors followed by transformation into competent cells. 

Benefits:

• Sequencing can provide valuable insight on the structure of you antibody. 
• Knowing the structure of your antibody can shed light on its interactions with other molecules.

Common applications:

• Patents – documenting your antibody sequence helps to protect your discovery.
• Differentiating antibodies made to a common target.

Technique:

At GMA, we employ a rigorous strategy to ensure that your antibody sequence is accurate!  We begin by isolating only cytoplasmic, processed RNA – free of genomic DNA contamination.  This is followed by a 5’ RACE method, allowing for amplification of the 5’ end of the variable region, which is traditionally difficult to prime.  This method, in combination with a set of nested PCR’s using gene specific primers extending from the constant region, results in complete variable region sequences.

Reliability:

All sequencing done at GMA has been crossed referenced with protein sequence obtained via Edman Degradation at an independent facility.  Sequences have matched 100%.  We also reference the guidelines provided by http://www.bioinf.org.uk/abs/ to locate and highlight the complementarity determining regions (CDR’s).  All sequences generated have contained the pattern of amino acids, or landmarks, most commonly surrounding the CDR’s.

Presentation:

Data is presented in both a nucleotide sequence and a protein sequence format. 

Your results will be emailed to you within 2-4 weeks.

Contact us.